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Method 2: Expression and Purification of M3-H18L6 (v37574) and M3-H1L1 (v36180) [00907] ExpiCHO TM cells were cultured at 37°C in ExpiCHO TM expression medium (Thermo Fisher Scientific™, Waltham, MA) on an orbital shaker rotating at 120 rpm in a humidified atmosphere of 8% CO 2 .100 mL expression volumes and 400 mL expression volume in the case of v37574 were used

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As humans age, pineal gland calcification and declining melatonin production disrupt circadian rhythms, impair sleep quality, suppress immune function, and accelerate oxidative damage