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According to this mechanism, the intracellular GSH/GSSG ratio dictates the extent of protein S-glutathionylation ([Protein-SSG]/[Protein-SH]), and the equilibrium constant for the reaction (K mix , Equation (1)) corresponds to the oxidation potential for the formation of the mixed disulfide (protein-SSG): Protein-SH + GSSG ⇌ Protein-SSG + GSH, The K mix value for most cysteine residues is approximately 1.0, so the GSH/GSSG ratio would have to be decreased greatly in order to favor the formation of protein-SSG [7]