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Samples containing identical amounts of protein were resolved using SDS-PAGE (Criterion, BioRad Laboratories), transferred to nitrocellulose, and blocked in Tris-buffered saline (TBS) containing 5% milk for one hour at room temperature
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And one of the most interesting stories in longevity science right now involves a peptide your own mitochondria are supposed to make — called MOTS-c — that appears to decline with age, drop sharply with estrogen, and play a surprisingly central role in how your body handles glucose, fat, and inflammation
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