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Until now, site-specific incorporation of phosphoserine (pSer) can be genetically encoded in mammalian cells in contrast to inabilities of phosphothreonine (pThr)/phosphotyrosine (pTyr) to be incorporated in mammalian cells, while GCE approaches have provided tremendous opportunities to incorporate them into recombinant proteins in E
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cDNA cloning, expression, and biochemical characterization
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