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Intranasal administration achieves the highest brain-to-plasma concentration ratios documented in any dihexa study: a 2021 pharmacokinetic analysis in Sprague-Dawley rats found that 0.5 mg/kg intranasal dihexa produced hippocampal tissue concentrations of 420 ng/g at 30 minutes post-administration, compared to 85 ng/g from 2.5 mg/kg subcutaneous injection at the same timepoint

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The body cannot produce B12 and therefore it is important to eat nutritious food so that the body can instead absorb vitamin B12 via the diet