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Cells with APEX2 or HRP were enzymatically reacted with 2.5 mM DAB (Sigma-Aldrich, D8001-10G) with 4 mM H 2 O 2 (from 30% stock) in 0.1 M sodium cacodylate buffer at pH 7.4 for 3–20 min until the desired brown intensity color of the precipitate was visible