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The cells were incubated overnight at 4 °C with the mouse anti-nestin antibody (1:200, Merck KGaA, Darmstadt, Germany), mouse anti-Tubulin βIII (1:200 Merck KGaA) and rabbit anti-glial fibrillary acidic protein (1:300 Dako Agilent Technologies, Santa Clara, CA, USA)
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14 (11), e1007802
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