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129,136,137,138,139,140 However, clinical translation of saRNAs remains challenging due to the absence of an immune system in xenograft models and the complexity of drug action in the human body
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Consequently, the maleimide functionality is mainly introduced via post-synthetic amide-couplings in solution-phase, where amino-modified oligonucleotide (AON) reacts with activated esters (see Fig
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