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50 ng/well of gp42 or gp350 were adsorbed directly on to 384 well microplates at 4 °C for 16 h in coating buffer, washed four times with ELISA wash buffer and blocked in blocking buffer as above

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730 FtMt protein expression is significantly upregulated in MCAO/R mice, and the absence of FtMt promotes free iron deposition and lipid peroxidation, which aggravates ferroptosis and brain injury caused by MCAO/R