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(The main function of the HPA axis is to release cortisol, one of your body’s stress hormones.)48 Ashwagandha appears to be the botanical ingredient that influences HPA activity the most, particularly when you take it in the morning.1 Your cortisol levels are the highest a few hours after waking up in the morning and then taper off all day, so lowering cortisol levels first means they should be lower all day, too