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While competition AlphaLISA and TR-FRET can both be applied to measure binary and ternary binding, these assay set-ups require a well-established labeled ‘probe’ ligand, and ternary complex formation parameters cannot be read directly, meaning that they have to be extracted by comparing ‘protein A’-degrader binding performed in the absence versus in the presence of ‘protein B’, which must assume binding saturation with protein B