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Although alterations in genetic factors have been linked with its etiology, exponential accumulation of environmental entities such as reactive oxygen species (ROS) initiate a cyclic chain reaction resulting in accumulation of cellular inclusions, dysfunctional mitochondria, and overwhelming of antioxidant machinery, thus accelerating disease pathogenesis

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The dried bacterial pellet was solubilized in 20 ml of B-PER (78243, Thermo Fisher Scientific), supplemented with protease inhibitors (1 µg ml −1 of leupeptin, 1 µg ml −1 of AEBSF HCl and 1 µl of benzonase) and 5 mM imidazole and then transferred to a 50-ml centrifuge tube