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The sample was redissolved in 100 μL of ice-cold 50% methanol, centrifuged again, and filtered through a 0.22-μm membrane to obtain the test solution
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Upon ligand binding, the c-Met intracellular tyrosine kinase domain undergoes autophosphorylation, initiating downstream signaling cascades including the Ras/MAPK, PI3K/Akt, and STAT3 pathways
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