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Lysates (∼90 μg of total protein per gel line) were seeded in a 10% SDS polyacrylamide gel and transferred to polyvinylidene difluoride membranes

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Structurally, the RBC cytoskeleton consists of α and β spectrin tetramers, which assemble into a pseudo-hexagonal lattice anchored to the plasma membrane by two main complexes (Figure 2): the ankyrin and the junctional complex, both stabilized by accessory proteins such as adducin, tropomodulin, and dematin, which form interconnected tethers approximately 80 nm apart [38,124]