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B-type Plexins promote the GTPase activity of Ran to affect androgen receptor nuclear translocation in prostate cancer | Cancer Gene Therapy

Although enhanced ER Ca²⁺ release could, in principle, influence mitochondrial Ca²⁺ uptake and downstream metabolic activation, mitochondrial Ca²⁺ levels were not directly measured in this study

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Afterwards, the cells were fixed with 4% paraformaldehyde to preserve their structural integrity, followed by a blocking step to minimize non-specific binding