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Cells were added at final density of OD 0.01 to the wells containing drugs and incubated at 37 °C for 3 days as described previously 48

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Further preclinical studies and clinical randomized controlled trials are needed to investigate and improve strategies for KD, such as optimization of the timing of interventions and nutrient composition, to assess the suitability, effectiveness, and safety of KD in the treatment of SUD