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NFS1 abstracts sulfur from free cysteine, and the subsequent reduction of sulfane sulfur (S 0 ) to sulphide (S 2− ) likely involves the concerted action of ferredoxin (FDX1 and FDX2 in humans [32]) and ferredoxin reductase (FDXR), which have been shown to provide electrons in prokaryotes [33]

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Cells were lysed with lysis buffer (20 mM Tris–HCl pH 8.0, 1% NP-40, 150 mM NaCl, 1 mM EDTA, and protease inhibitor cocktail) and lysates were centrifuged at 16,000× g for 20 min at 4 °C