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EGM-2 (Lonza, Basel, Switzerland) was supplemented with the following substances: 0.4% vascular endothelial growth factor (VEGF), 0.4% human basic fibroblast growth factor (hFGF-B), 0.1% recombinant analogue of human insulin-like growth factor-I (R3-IGF-1), 0.1% ascorbic acid, 0.1% human epidermal growth factor (hEGF), and 0.1% Gentamicin Sulphate-Amphotericin (GA-1000) and 0.1% heparin [34]