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glutathione and pulmonary hypertension Frontiers Molecular Mechanisms of Iron

Molecular Mechanisms of Iron Metabolism and Overload - PMC

Features of Glutathione Agarose: • High capacity —binds at least 40 mg of purified recombinant GST protein per milliliter of resin • High yield and purity —consistently purifies at least 10 mg of GST-tagged protein per milliliter of resin with greater than 90% purity • Cost-effective —resin is economically priced and can be reused at least five times without reduction in binding capacity and purification performance • Versatile —works well to purify GST-fusion proteins from bacterial lysates or use with pre-purified GST-tagged proteins to pull down protein interactions • Compatible —validated and effective for use with Thermo Scientific Cell Lysis Reagents to extract and purify from bacterial or mammalian cell cultures The glutathione is immobilized through its central sulfhydryl onto 6% crosslinked agarose resin

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glutathione and pulmonary hypertension Frontiers Molecular Mechanisms of Iron

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