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After treatment, media was removed and cells rinsed in PBS before lysis with 200 μl ice-cold RIPA buffer (Sigma, UK) containing protease inhibitors (Complete EDTA protease inhibitor tablets

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BPC-157 cannot legally be sold as a dietary supplement ingredient under FDA rules, and products labeled 'for research purposes only' or 'research chemicals' are marketing loopholes that do not indicate safety or approval for human use