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Reverse phase liquid chromatography-quadrupole-time of flight mass spectrometry (RP/HPLC-qTOF MS/MS) analysis Aliquots of 2 μL (for both ESI (+) and ESI (−) modes) were injected into a LC–MS/MS system consisting of a quadrupole Q-TOF series 6540 coupled to a HPLC (model 1290) both from Agilent Technologies (Germany), equipped with an Agilent Jet Stream (AJS) thermal orthogonal ESI source
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