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On average, humans synthesize 0.4 mmol to 1.0 mmol (50 mg to 125 mg) of taurine daily (115)
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For luciferase reporter vectors, PRKAA1 5′UTR or scramble 5′UTR DNA sequences were synthesized and cloned into pCDNA3 RLuc POLIRES FLuc (Addgene, 45642)
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