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After Rut intervention, it significantly reduced the oxidative stress of cells, promoted the translocation of nuclear factor red cell 2 related factor 2 (Nrf2) from the cytoplasm to the nucleus, and then activated the expression of antioxidant substances including Heme Oxygenase-1 (HO-1), and knocked out Nrf2 by small interfering RNA (siRNA) transfection to reduce the protective effect of Rut on lead induced cells, suggesting that Rut alleviated lead induced oxidative stress by activating Nrf2/ARE pathway in SH-SY5Y cells and played its antioxidant role (Li et al
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Plasma figures reported in a 2015 review A 2015 review by Pickart and colleagues in BioMed Research International (PMID 26236730) reports plasma GHK at about 200 nanograms per millilitre near age 20 and about 80 nanograms per millilitre near age 60
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