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"Brain-gut axis and pentadecapeptide BPC 157: theoretical and practical implications." Curr Neuropharmacol
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Tissue homogenate was processed for RNA extraction, followed by reverse transcription (for cDNA synthesis) and quantitative real-time PCR to quantify AMP-activated protein kinase (AMPK) and aging-related pathway (p53/p21/p16) gene expression using kits from Thermo Fisher Scientific, USA
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