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Furthermore, regarding the clinically prevalent issue of bovine mastitis, the core volatile oil component of HC, 2-methylnonyl ketone (MNK), protected bovine mammary epithelial cells from LPS-induced oxidative damage and inflammatory injury by inhibiting the TLR4-NF-κB signaling pathway and activating the Nrf2/HO-1 pathway (41)
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A cell-penetrating peptide mimicking this region disrupted the FOXO3-p300 interaction in cells (IC 50 = 8 μM), reduced target gene HIF-1α expression by 45%, and inhibited tumor growth in a xenograft model by 52%
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