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Vitamin B12: Fact Sheet for Consumers [Internet]
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In order to determine the internal control (β-actin), the semi-quantitative RT-PCR was performed using the following temperature protocol: Denaturation at 94°C for 30 sec, annealing at 56°C for 30 sec, extension at 72°C for 25 sec for 30 cycles, and a final extension at 72°C for 4 min
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