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In contrast, significant increases in the CD3- and CD4-positive peripheral blood cells and enhancement of stimulated lymphocyte proliferation have been observed after long-term treatment with methotrexate [83], and adenosine, acting through and A 2A A 2B receptors, may play a role in T-cell deactivation [84,85]

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In contrast to the findings of Yan et al., our study did not show a decrease in blood insulin levels, likely because our data were obtained from an OGTT