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chimeric proteins refers to proteins in which an antigen binding domain is from a particular species (e.g., murine, such as mouse or rat) or belonging to a particular antibody class or subclass, while the remainder of the protein is from a protein derived from another species (such as, for example, human or non-human primate) or belonging to another antibody class or subclass

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Tandem affinity purification results in more highly refined and stable complexes than single-tagged strategy alone, as this technique requires even more strict binding and elution conditions