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The surface of microglia contains a number of receptors, including receptors for most of the neurotransmitters, pro- and anti-inflammatory cytokines, chemokines, interferons, and major histocompatibility complex Classes I and II receptors.[15] Microglia also contain characteristic receptors called pattern recognition receptors (PRR), which are constitutively expressed to identify and bind various pathogen-associated molecular pattern sites and other non-self-molecules.[15] In addition, microglia also express toll-like receptors (TLRs), with TLR 1–9 of the 12 of the known TLRs being found on microglia membranes.[116] TLRs not only recognize microbial antigens but also regulate the magnitude and duration of the immune response

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The middle of this range, around 4°C (39°F), provides optimal balance between preservation and avoiding accidental freezing